Difference between revisions of "Part:BBa K782087"

Revision as of 11:30, 26 September 2012

10x[TALA] operator_minimal promoter

• TALA and TALC labels represents TAL effector 1257 and 1259 from zebrafish experiments (Sander et al., 2011).

Introduction

TAL effectors (TALEs) are able to specifically bind to the DNA by recognizing base pairs on a DNA molecule. With TAL regulators we are able to control expression of a desired gene. Efficient transcription control can be achieved by binding of multiple TAL regulators upstream of promoter.

Our construct contain ten TALA binding sites and minimal promoter.

Minimal promotor is taken from pGL4 vector series (promotor is described [http://www.promega.com/~/media/files/resources/protocols/technical%20manuals/0/pgl4%20luciferase%20reporter%20vectors%20protocol.pdf?la=en here] on the page 18).

Single binding site is combined from 18 base pairs specificly recognised by TALA. Binding sites are constructed in alternating setup. Besides TALA binding sites construct also contain ten alternating TALC binding sites, which we didn't use, since TALC regulator wasn't working properly.

Single binding site sequence for TALA: TTTACTGCTGCTCCCGCT

Figure 1: Schematic representation of the construct.

Sequence and Features