Difference between revisions of "Part:BBa K782079"

Revision as of 21:13, 25 September 2012

10x[TALA] operator_CMV promoter_2xBFP:NLS

TALA labels represents TAL effector 1257 from zebrafish experiments (Sander et al., 2011).

Introduction

Our construct contain TALA binding sites that are cloned downstream of CMV promoter. Upstream of CMV is a double blue fluorescent protein with NLS domain, causing protein to migrate into the nucleus. BFP is a monomeric fluorescent protein with excitation maximum at 402 nm and emision maximum at 457 nm.

Figure 1: Schematic representation of the construct.

Characterization

Results:

BFP was obtained from Evrogen.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 720
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 147
Illegal NgoMIV site found at 507
Illegal AgeI site found at 12
Illegal AgeI site found at 347
Illegal AgeI site found at 372
Illegal AgeI site found at 707
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 1396
Illegal BsaI.rc site found at 2052
Illegal BsaI.rc site found at 2757

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K782079 short</partinfo>
-long
+TALA labels represents TAL effector 1257 from zebrafish experiments (Sander et al., 2011).
+==Introduction==
+Our construct contain [https://parts.igem.org/Part:BBa_K782070 TALA binding sites] that are cloned downstream of CMV promoter. Upstream of CMV is a double blue fluorescent protein with NLS domain, causing protein to migrate into the nucleus. BFP is a monomeric fluorescent protein with excitation maximum at 402 nm and emision maximum at 457 nm.
+[[Image:Abfp2.png‎]]
+'''Figure 1:''' Schematic representation of the construct.
+==Characterization==
+Results:
+BFP was obtained from Evrogen.
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