Difference between revisions of "Part:BBa K929100"
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! colspan="2" style="background:#66bbff;"|[https://parts.igem.org/Part:BBa_K929000 Anti-EGFR scFv425
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! colspan="2" style="background:#66bbff;"|[https://parts.igem.org/Part:BBa_K929000 Anti-EGFR scFv425]
 
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Revision as of 09:48, 24 September 2012

Anti-EGFR scFv425

Anti-EGFR scFv425
[[Image:|300px]]
BioBrick Nr. BBa_929100
RFC standard RFC 25
Requirement pSB1C3
Source plasmid pKMEF425bla
Submitted by [http://2012.igem.org/Team:Potsdam_Bioware Potsdam_Bioware2012]


File:.png
400px




















The single chain fragment (scFV425) specifically binds to the epidermal growth factor receptor (EGFR) domain 3. The part has a shortened N-terminal FLAG-tag sequence of five amino acids (DYKDE) for purification and detection.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 538
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]