Difference between revisions of "Part:BBa K897318"
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__NOTOC__
 
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<partinfo>BBa_K897318 short</partinfo>
 
<partinfo>BBa_K897318 short</partinfo>
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  As we know, all antisense RNAs had a short half-life of about 1 minute. Because antisense RNA  
+
  As we know, all antisense RNAs had a short half-life of about 1 minute. Because antisense RNA efficiency is determined by the  
−
efficiency is determined by the binding rate to the target, which in turn is determined by both  
+
binding rate to the target, which in turn is determined by both antisense RNA concentration and binding rate constant, a higher  
−
antisense RNA concentration and binding rate constant, a higher intracellular concentration of  
+
intracellular concentration of the inhibitor will result in higher efficacy.
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the inhibitor will result in higher efficacy.
+
   An alternative strategy to stabilize asRNAs is to pair the termini using flanking inverted repeats to create a hairpin structure
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   An alternative strategy to stabilize asRNAs is to pair the termini using flanking inverted  
+
with the antisense sequence within a large loop. A paired termini (PT) design, where flanking inverted repeats create paired asRNA  
−
repeats to create a hairpin structure with the antisense sequence within a large loop. A  
+
termini, was proved can produce effective gene silencing. PT asRNAs are abundant and stable and function through an RNase III  
−
paired termini (PT) design, where flanking inverted repeats create paired asRNA termini, was  
+
independent mechanism that requires a large stoichiometric excess of asRNA.PT consists of non-endogenous GC-rich sequences and has
−
proved can produce effective gene silencing. PT asRNAs are abundant and stable and function  
+
a stem-loop structure, which can improve the stability of as RNA raises its abundance.
−
through an RNase III independent mechanism that requires a large stoichiometric excess of asRNA.
+
−
PT consists of non-endogenous GC-rich sequences and has a stem-loop structure, which can improve
+
−
the stability of as RNA raises its abundance.
+
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===

Revision as of 04:48, 24 September 2012

Paired termini structure 

As we know, all antisense RNAs had a short half-life of about 1 minute. Because antisense RNA efficiency is determined by the

binding rate to the target, which in turn is determined by both antisense RNA concentration and binding rate constant, a higher intracellular concentration of the inhibitor will result in higher efficacy. 

 An alternative strategy to stabilize asRNAs is to pair the termini using flanking inverted repeats to create a hairpin structure
with the antisense sequence within a large loop. A paired termini (PT) design, where flanking inverted repeats create paired asRNA

termini, was proved can produce effective gene silencing. PT asRNAs are abundant and stable and function through an RNase III independent mechanism that requires a large stoichiometric excess of asRNA.PT consists of non-endogenous GC-rich sequences and has 

a stem-loop structure, which can improve the stability of as RNA raises its abundance.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 59
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 59
    Illegal NotI site found at 84
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 59
    Illegal BamHI site found at 53
    Illegal XhoI site found at 93
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 59
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 59
  • 1000
    COMPATIBLE WITH RFC[1000]