Difference between revisions of "Part:BBa K897720"
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In order to interfere with the cell division, we synthesize the right antisense fragments with gene silencing effects targeting ftsZ | In order to interfere with the cell division, we synthesize the right antisense fragments with gene silencing effects targeting ftsZ | ||
in E.coli. A vector with paired termini was constructed to constantly express different asRNAs of ftsZ in E.coli,and this part has | in E.coli. A vector with paired termini was constructed to constantly express different asRNAs of ftsZ in E.coli,and this part has | ||
| − | + | been found inhibiting cell division considerbly. | |
Revision as of 02:59, 24 September 2012
Antisense FtsZ protected by paired termini structure under T7 promoter
In order to interfere with the cell division, we synthesize the right antisense fragments with gene silencing effects targeting ftsZ in E.coli. A vector with paired termini was constructed to constantly express different asRNAs of ftsZ in E.coli,and this part has been found inhibiting cell division considerbly.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 65
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 261
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 65
- 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 65
- 1000COMPATIBLE WITH RFC[1000]