Difference between revisions of "Part:BBa K838000"

Revision as of 16:40, 26 September 2012

Mammalian LovTAP-VP16

LovTAP-VP16 is a light-sensitive protein that binds to DNA. The protein is a fusion between a Lov light activated domain, a TRP repressor, an NLS/ linker domain and a VP16 activator domain. As it contains a VP16 activational domain, it's able to act as a transcriptional activator when it is bound to a TRP promoter site.

The lov domain clashes with the TRP repressor domain and prevents binding before the protein is exposed to blue light. After light activation the Lov domain goes through a comformational change and the TRP repressor domain of the protein can bind DNA.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal XbaI site found at 274
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 772
Illegal XhoI site found at 817
23
INCOMPATIBLE WITH RFC[23]
Illegal XbaI site found at 274
25
INCOMPATIBLE WITH RFC[25]
Illegal XbaI site found at 274
1000
COMPATIBLE WITH RFC[1000]

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K838000 short</partinfo>
-LovTAP-VP16 is a light-sensitive protein that binds to DNA. As it contains a VP16 activational domain, it's able to act as an activator
+LovTAP-VP16 is a light-sensitive protein that binds to DNA. The protein is a fusion between a Lov light activated domain, a TRP repressor, an NLS/ linker domain and a VP16 activator domain. As it contains a VP16 activational domain, it's able to act as a transcriptional activator when it is bound to a TRP promoter site.
+The lov domain clashes with the TRP repressor domain and prevents binding before the protein is exposed to blue light. After light activation the Lov domain goes through a comformational change and the TRP repressor domain of the protein can bind DNA.
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