Difference between revisions of "Part:BBa K895007:Experience"
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| + | ===Results=== | ||
| + | <i>E. coli</i> MG1655 was transformed with <partinfo>BBa_K895007</partinfo> grown aerobically overnight in LB medium. The culture was then used to inoculate 30 ml anaerobic cultures in LB only, LB + 0.4% (w/v) tetrathionate or LB + 0.4% (w/v) thiosulfate. Following 16 hours growth aliquotes were taken and analysed by SDS-PAGE and Western immunoblotting using an anti-GFP monoclonal. | ||
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| + | This small scale aassay reveals that GFP was only expressed from the plasmid in the presence of tetrathionate. The device therefore works as a putative <b>inflammation biosensor</b>. | ||
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| + | [[Image:Dundee_Biosensor_Figure5.jpg]] | ||
| + | :Figure 1: Production of GFP from <partinfo>BBa_K895007</partinfo> is tetrathionate dependent. The RED ARROW points to the lane producing GFP. | ||
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Revision as of 21:35, 26 September 2012
This experience page is provided so that any user may enter their experience using this part.
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UNIQ7f3dd6a31326cec2-partinfo-00000000-QINU UNIQ7f3dd6a31326cec2-partinfo-00000001-QINU