Difference between revisions of "Part:BBa K823051"

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far-red monomeric fluorscent protein in Freiburg standard (RCF 25), with RBS and Terminator (B0014).
 
far-red monomeric fluorscent protein in Freiburg standard (RCF 25), with RBS and Terminator (B0014).
 +
 +
Find out more about the design of our [https://static.igem.org/mediawiki/parts/b/b9/LMU-Munich_2012_Our_Freiburg_standard_FusionPrefix.pdf prefix with ribosome binding site].
  
 
prefix:GAATTCCGCGGCCGCTTCTAGATAAGGAGGAACTACTATGGCCGGC
 
prefix:GAATTCCGCGGCCGCTTCTAGATAAGGAGGAACTACTATGGCCGGC

Revision as of 08:56, 26 September 2012

mKate2, a red monomeric fluorescent protein + RBS + Term

far-red monomeric fluorscent protein in Freiburg standard (RCF 25), with RBS and Terminator (B0014).

Find out more about the design of our prefix with ribosome binding site.

prefix:GAATTCCGCGGCCGCTTCTAGATAAGGAGGAACTACTATGGCCGGC

suffix:ACCGGTTAATACTAGTAGCGGCCGCTGCAGT

Excitation maximum: 588 nm

Emission maximum: 633 nm

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 4
  • 1000
    COMPATIBLE WITH RFC[1000]