Difference between revisions of "Part:BBa K785003"

Latest revision as of 13:11, 26 September 2012

Lux(CDABEG) coding gene PoPS->Light

This page described the Lux operon from Vibrio fischeri. Lux gene is directly PCRed from the BBa_K325909, the pbad operon is deleted and now this part is available for any expression unit. You can provide light out put by putting this coding device under your promoter. We have successfully put this coding sequence into the PET28a expression system (cell strain is BL21 DE3), and light out put could be induced by the IPTG. To see more information about Lux light generator, please visit BBa_K325909, and a more complete description can be found on the [http://2010.igem.org/Team:Cambridge/Bioluminescence/G28 2010 Cambridge iGEM].

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 2961
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 1959
Illegal XhoI site found at 2789
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 4348
Illegal BsaI.rc site found at 1357
Illegal SapI.rc site found at 4673

The Lux light generator part expressed in the PET28a system(under the T7 lacO control). The Lux is induced by IPTG.

@@ Line 3: / Line 3: @@
 This page described the Lux operon from Vibrio fischeri. Lux gene is directly PCRed from the BBa_K325909, the pbad operon is deleted and now this part is available for any expression unit. You can provide light out put by putting this coding device under your promoter.
-We have successfully put this coding sequence into the PET28a expression system (cell strain is dH5&#945;), and light out put could induced by the IPTG.
+We have successfully put this coding sequence into the PET28a expression system (cell strain is BL21 DE3), and light out put could be induced by the IPTG.
 To see more information about Lux light generator, please visit [https://parts.igem.org/Part:BBa_K325909 BBa_K325909], and a more complete description can be found on the [http://2010.igem.org/Team:Cambridge/Bioluminescence/G28 2010 Cambridge iGEM].