Difference between revisions of "Part:BBa K743008"
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It was used by [http://2012.igem.org/Team:UC_Chile UC_Chile 2012 team] to succesfully transform Synechocystis | It was used by [http://2012.igem.org/Team:UC_Chile UC_Chile 2012 team] to succesfully transform Synechocystis | ||
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'''Verification Colony PCR''' | '''Verification Colony PCR''' | ||
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'''psb1C3_IntK plasmid with BBa_K743008 digested with EcoR1 and Pst1''' | '''psb1C3_IntK plasmid with BBa_K743008 digested with EcoR1 and Pst1''' | ||
Revision as of 23:42, 22 September 2012
psb1C3_IntKR recombination plasmid for Synechocystis PCC6803
This plasmid allows the integration of a double terminator followed by a reversed Kanamycin resistance casette in to Synechocystis chromosome, making possible selection in this cyanobacteria. It has a Chloramphenicol resistance casette for selection in E.coli The use of BBa_K743000 and BBa_K743001 as recombination sites has no deleterious phenotypic effects on the cells. The plasmid is intended to be used as a starting backbone for further adition of dna parts by Gibson assembly. It was used by [http://2012.igem.org/Team:UC_Chile UC_Chile 2012 team] to succesfully transform Synechocystis
Verification Colony PCR
psb1C3_IntK plasmid with BBa_K743008 digested with EcoR1 and Pst1
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1758
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1758
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1758
Illegal XhoI site found at 2255 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1758
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1758
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 511