Difference between revisions of "Part:BBa K743008"

Revision as of 23:22, 22 September 2012

psb1C3_IntKR recombination plasmid for Synechocystis PCC6803

This plasmid allows the integration of a double terminator followed by a reversed Kanamycin resistance casette in to Synechocystis chromosome, making possible selection in this cyanobacteria. It has a Chloramphenicol resistance casette for selection in E.coli The use of BBa_K743000 and BBa_K743001 as recombination sites has no deleterious phenotypic effects on the cells. The plasmid is intended to be used as a starting backbone for further adition of dna parts by Gibson assembly. It was used by [http://2012.igem.org/Team:UC_Chile UC_Chile 2012 team] to succesfully transform Synechocystis

Verification Colony PCR

psb1C3 plasmid with BBa_K743008 digested with EcoR1 and Pst1

Sequence and Features