Difference between revisions of "Part:BBa K743008"
Juan Alamos (Talk | contribs) |
Juan Alamos (Talk | contribs) |
||
Line 7: | Line 7: | ||
It was used by [http://2012.igem.org/Team:UC_Chile UC_Chile 2012 team] to succesfully transform Synechocystis | It was used by [http://2012.igem.org/Team:UC_Chile UC_Chile 2012 team] to succesfully transform Synechocystis | ||
− | [[Image:gelC4_UC_Chile.jpg]] | + | [[Image:gelC4_UC_Chile.jpg|400px]] |
+ | |||
+ | [[Image:Example.jpg]] | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here |
Revision as of 23:17, 22 September 2012
psb1C3_IntKR recombination plasmid for Synechocystis PCC6803
This plasmid allows the integration of a double terminator followed by a reversed Kanamycin resistance casette in to Synechocystis chromosome, making possible selection in this cyanobacteria. It has a Chloramphenicol resistance casette for selection in E.coli The use of BBa_K743000 and BBa_K743001 as recombination sites has no deleterious phenotypic effects on the cells. The plasmid is intended to be used as a starting backbone for further adition of dna parts by Gibson assembly. It was used by [http://2012.igem.org/Team:UC_Chile UC_Chile 2012 team] to succesfully transform Synechocystis
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1758
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1758
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1758
Illegal XhoI site found at 2255 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1758
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1758
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 511