Difference between revisions of "Part:BBa K743016:Design"

 
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__NOTOC__
 
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<partinfo>BBa_K743016 short</partinfo>
 
<partinfo>BBa_K743016 short</partinfo>
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===Source===
 
===Source===
  
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psbA2 was amplified from Synechocystis genomic DNA and its sequence was obtained from BBa_K390017.
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psbA2 was amplified from Synechocystis genomic DNA and its sequence was obtained from [https://parts.igem.org/Part:BBa_K390017 BBa_K390017].
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RFP is from blubliblo
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RFP is from [https://parts.igem.org/Part:BBa_E1010 BBa_E1010].
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For more information about the plasmid backbone, check [https://parts.igem.org/Part:BBa_K743008 psb1C3_IntKR recombination plasmid] page.
 
For more information about the plasmid backbone, check [https://parts.igem.org/Part:BBa_K743008 psb1C3_IntKR recombination plasmid] page.
  
 
===References===
 
===References===

Latest revision as of 22:37, 22 September 2012

psb1C3_IntKR recombination plasmid with RFP under psbA2 promoter


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2551
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2551
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2551
    Illegal XhoI site found at 3048
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2551
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2551
    Illegal AgeI site found at 1164
    Illegal AgeI site found at 1276
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 511


Design Notes

This composite part is not intended to be used for standard biobrick assembly because of a EcoR1 restriction site in psb1C3_intK plasmid. You should use gibson assembly.


Source

psbA2 was amplified from Synechocystis genomic DNA and its sequence was obtained from BBa_K390017. RFP is from BBa_E1010.

For more information about the plasmid backbone, check psb1C3_IntKR recombination plasmid page.

References