Difference between revisions of "Part:BBa K863101:Design"

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===Design Notes===
 
===Design Notes===
 
The sequence starts with the rest of the Freiburg-prefix (ATG and ''Ngo''MIV-restriction-site). The A from the startcodon ATG is the last base of the ''Xba''I restriction-site of the Standard [https://parts.igem.org/Assembly_standard_25 25 Prefix (Read here)]. I isolated 12 conserved Bases (4 AS) upstream of the cellulose binding domain and 9 bases downstream of the exoglucanase gene and added a short N-terminal (Glycine, Serine) linker. The sequence ends with the ''Age''I-restriction-site of the Freiburg-Suffix, to make in frame assembly possible.
 
The sequence starts with the rest of the Freiburg-prefix (ATG and ''Ngo''MIV-restriction-site). The A from the startcodon ATG is the last base of the ''Xba''I restriction-site of the Standard [https://parts.igem.org/Assembly_standard_25 25 Prefix (Read here)]. I isolated 12 conserved Bases (4 AS) upstream of the cellulose binding domain and 9 bases downstream of the exoglucanase gene and added a short N-terminal (Glycine, Serine) linker. The sequence ends with the ''Age''I-restriction-site of the Freiburg-Suffix, to make in frame assembly possible.
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 +
{|
 +
|CBDcex_Freiburg-Prefix||GCTAGAATTCGCGGCCGCTTCTAGATGGCCGGCGGTCCGGCCGGGTGCCAGGTG
 +
|-
 +
|CBDcex_2AS-Link_Frei||CTGCAGCGGCCGCTACTAGTATTAACCGGTGCTGCCGCCGACCGTGCAGGGCGTGC
 +
|}
  
 
===Source===
 
===Source===

Revision as of 19:33, 26 September 2012

Cellulose binding Domain of Cellulomonas Fimi Exoglucanse (Freiburg-Standard)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 4
    Illegal AgeI site found at 337
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence starts with the rest of the Freiburg-prefix (ATG and NgoMIV-restriction-site). The A from the startcodon ATG is the last base of the XbaI restriction-site of the Standard 25 Prefix (Read here). I isolated 12 conserved Bases (4 AS) upstream of the cellulose binding domain and 9 bases downstream of the exoglucanase gene and added a short N-terminal (Glycine, Serine) linker. The sequence ends with the AgeI-restriction-site of the Freiburg-Suffix, to make in frame assembly possible.

CBDcex_Freiburg-Prefix GCTAGAATTCGCGGCCGCTTCTAGATGGCCGGCGGTCCGGCCGGGTGCCAGGTG
CBDcex_2AS-Link_Frei CTGCAGCGGCCGCTACTAGTATTAACCGGTGCTGCCGCCGACCGTGCAGGGCGTGC

Source

Cloning-Vector with a CBDcex-Barnase fusion-protein

References