Difference between revisions of "Part:BBa K733018:Design"
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===Source=== | ===Source=== | ||
| − | We digest and ligate our [https://parts.igem.org/Part:BBa_K733002 xylR+PxylA] and [https://parts.igem.org/Part:BBa_E0240 BBa_E0240] from 2012 Kit Plate. | + | We digest and ligate our [https://parts.igem.org/Part:BBa_K733002 ''xylR''+''PxylA''] and [https://parts.igem.org/Part:BBa_E0240 BBa_E0240] from 2012 Kit Plate. |
| − | Backbone: xylR+PxylA in pSB1C3. Enzymes used: SpeI and PstI-HF. | + | Backbone: ''xylR''+''PxylA'' in pSB1C3. Enzymes used: SpeI and PstI-HF. |
Insert: BBa_E0240. Enzymes used: XbaI and PstI-HF. Purified by gel puficiation. | Insert: BBa_E0240. Enzymes used: XbaI and PstI-HF. Purified by gel puficiation. | ||
===References=== | ===References=== | ||
Latest revision as of 17:02, 26 September 2012
xylR+PxylA+RBS+GFP+Double Terminator
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 847
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 2058
Design Notes
None.
Source
We digest and ligate our xylR+PxylA and BBa_E0240 from 2012 Kit Plate.
Backbone: xylR+PxylA in pSB1C3. Enzymes used: SpeI and PstI-HF.
Insert: BBa_E0240. Enzymes used: XbaI and PstI-HF. Purified by gel puficiation.