Difference between revisions of "Part:BBa K733002"
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This part is extracted by PCR from pAX01 plasmid. （Zeigler 2002）In the region of PxylA, there are two promoters with opposite directions: one regulates the expression of xylR, and another one is regulated by xylR.  (Rygus et al., 1991) When xylR is expressed, it will inhibit the expression of the gene on the downstream of PxylA. However, without xylR, PxylA can be regarded as a constitutive promoter. Besides, upon the induction of xylose, the transcriptional regulator will not bind to the binding site in the promoter region, and transcription will start.
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This part is extracted by PCR from pAX01 plasmid. （Zeigler 2002）In the region of PxylA, there are two promoters with opposite directions: one regulates the expression of xylR, and another one is regulated by xylR.  (Rygus et al., 1991) When xylR is expressed, it will generate XylR, inhibiting the expression of the gene on the downstream of PxylA. However, without xylR, PxylA can be regarded as a constitutive promoter. Besides, upon the induction of xylose, the transcriptional regulator will not bind to the binding site in the promoter region, and transcription will start.
  
 
[[Image:Pxyl1.png]]  [[Image:Pxyl2.png]]
 
[[Image:Pxyl1.png]]  [[Image:Pxyl2.png]]
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Zeigler, D. (2002). Integration Vectors for Gram-Positive Bacteria (7 ed.). Columbus: The Bacillus Genetic Stock Center.
 
Zeigler, D. (2002). Integration Vectors for Gram-Positive Bacteria (7 ed.). Columbus: The Bacillus Genetic Stock Center.
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Rygus, T., Scheler, A., Allmansberger, R., & Hillen, W. (1991). Molecular cloning, structure, promoters and regulatory elements for transcription of the Bacillus megaterium encoded regulon for xylose utilization. Archives of Microbiology, 155, 535-542.
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Rygus, T., Scheler, A., Allmansberger, R., & Hillen, W. (1991). Molecular cloning, structure, promoters and regulatory elements for transcription of the Bacillus megaterium encoded regulon for xylose utilization. ''Archives of Microbiology'', 155, 535-542.

Revision as of 17:12, 22 September 2012

xylR+PxylA: A xylose inducible promoter with its transcriptional regulator.


This part is extracted by PCR from pAX01 plasmid. （Zeigler 2002）In the region of PxylA, there are two promoters with opposite directions: one regulates the expression of xylR, and another one is regulated by xylR. (Rygus et al., 1991) When xylR is expressed, it will generate XylR, inhibiting the expression of the gene on the downstream of PxylA. However, without xylR, PxylA can be regarded as a constitutive promoter. Besides, upon the induction of xylose, the transcriptional regulator will not bind to the binding site in the promoter region, and transcription will start.

Pxyl1.png Pxyl2.png


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 847
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]



Reference

Zeigler, D. (2002). Integration Vectors for Gram-Positive Bacteria (7 ed.). Columbus: The Bacillus Genetic Stock Center. Rygus, T., Scheler, A., Allmansberger, R., & Hillen, W. (1991). Molecular cloning, structure, promoters and regulatory elements for transcription of the Bacillus megaterium encoded regulon for xylose utilization. Archives of Microbiology, 155, 535-542.