Difference between revisions of "Part:BBa K743013"

Latest revision as of 20:03, 24 September 2012

Arabinose --> HIV protease secretion

OmpA - HIV autocleavage secretion system

Upon addition of arabinose to the medium, the inducible Pbad promoter drives the expression of outer membrane protein A fused to a extracellular linker (ompA brick) followed by a cleavage site recognizable by HIV protease which is linked to that protease coding sequence.

We expect the protease recognizes its own cleavage site and releases itself to the medium.

This part was used by [http://2012.igem.org/Team:UC_Chile 2012 UC Chile team] to secrete GFP.

You can check the results HERE LINK!

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 1205
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 1144
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 979
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI site found at 961

@@ Line 4: / Line 4: @@
 '''OmpA - HIV autocleavage secretion system'''
-Upon addition of arabinose to the medium, the inducible Pbad promoter drives the expression of outer membrane protein A  fused to a extracellular linker ([https://parts.igem.org/Part:BBa_K103006 ompA brick]) followed by a [https://parts.igem.org/Part:BBa_I712015 cleavage site] recognizable by [https://parts.igem.org/Part:BBa_I712667 HIV protease] wich is linked to that protease coding sequence.
+Upon addition of arabinose to the medium, the inducible Pbad promoter drives the expression of outer membrane protein A  fused to a extracellular linker ([https://parts.igem.org/Part:BBa_K103006 ompA brick]) followed by a [https://parts.igem.org/Part:BBa_I712015 cleavage site] recognizable by [https://parts.igem.org/Part:BBa_I712667 HIV protease] which is linked to that protease coding sequence.
-We expect for the protease to recognize it´s own cleavage site and to be released to the medium.
+We expect the protease recognizes its own cleavage site and releases itself to the medium.
+This part was used by [http://2012.igem.org/Team:UC_Chile 2012 UC Chile team] to secrete GFP.
-It was used by [http://2012.igem.org/Team:UC_Chile 2012 UC Chile team] to secrete GFP.
 You can check the results HERE LINK!