Difference between revisions of "Part:BBa K743013"

Revision as of 16:33, 22 September 2012

Arabinose --> HIV protease secretion

OmpA - HIV autocleavage secretion system

Upon addition of arabinose to the medium, the inducible Pbad promoter drives the expression of outer membrane protein A fused to a extracellular linker (ompA brick) followed by a cleavage site recognizable by HIV protease wich is linked to that protease coding sequence.

We expect for the protease to recognize it´s own cleavage site and to be released to the medium.

It was used by [http://2012.igem.org/Team:UC_Chile 2012 UC Chile team] to secrete GFP. You can check the results HERE LINK!

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 1205
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 1144
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 979
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI site found at 961

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K743013 short</partinfo>
+'''OmpA - HIV autocleavage secretion system'''
+Upon addition of arabinose to the medium, the inducible Pbad promoter drives the expression of outer membrane protein A  fused to a extracellular linker ([https://parts.igem.org/Part:BBa_K103006 ompA brick]) followed by a [https://parts.igem.org/Part:BBa_I712015 cleavage site] recognizable by [https://parts.igem.org/Part:BBa_I712667 HIV protease] wich is linked to that protease coding sequence.
+We expect for the protease to recognize it´s own cleavage site and to be released to the medium.
+It was used by [http://2012.igem.org/Team:UC_Chile 2012 UC Chile team] to secrete GFP.
+You can check the results HERE LINK!