Difference between revisions of "Part:BBa K896006:Design"
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Cloning of NirN-NirS gene: | Cloning of NirN-NirS gene: | ||
SQR gene came from Synechococcus elongatus PCC7002 because the hypersaline strain S. elongatus PCC 7002, which is already sequenced, is 96% similar to O.limnetica SQR gene.<p></p> | SQR gene came from Synechococcus elongatus PCC7002 because the hypersaline strain S. elongatus PCC 7002, which is already sequenced, is 96% similar to O.limnetica SQR gene.<p></p> | ||
| − | [[Image:NirN | + | [[Image:NirN+S cloning.jpg]] |
===Source=== | ===Source=== | ||
Latest revision as of 12:40, 22 September 2012
NirN-NirS (Nitrite->Nitric oxide reductase)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1703
Illegal BamHI site found at 1483 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 52
Illegal NgoMIV site found at 168
Illegal NgoMIV site found at 220
Illegal NgoMIV site found at 604
Illegal NgoMIV site found at 1297
Illegal NgoMIV site found at 2305
Illegal AgeI site found at 3169 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 2347
Illegal BsaI.rc site found at 2479
Design Notes
Cloning of NirN-NirS gene:
SQR gene came from Synechococcus elongatus PCC7002 because the hypersaline strain S. elongatus PCC 7002, which is already sequenced, is 96% similar to O.limnetica SQR gene.
Source
YES