Difference between revisions of "Part:BBa K861140"
Line 9: Line 9:
 
</p>
 
</p>
 
</html>
 
</html>
 +
===Characterized by XJTU-China 2020===
 +
We cloned and confirmed the target fragment by PCR. The length of our fragment is 981bp. The following results showed the successful result.
 +
<div>[[File:T--XJTU-China--Eggel.png |700px|thumb|center|<b>Figure 1:</b>PCR confirmation of the target fragment.]]</div>
  
 +
<div>[[File:T--XJTU-China--Bgimprove02.jpeg |700px|thumb|center|]]</div>
 +
<div>[[File:T--XJTU-China--Bgimprove01.jpeg |700px|thumb|center|]]</div>
 +
the mRNA level of B.s GalU is ~2.4 fold higher than that of E.coli, indicating the significant improvement of enzyme expression and activity. However, due to the lower expression of another key enzyme PGM for EPS production in BE strain, the final EPS yield is lower than that of EE strain but still comparable.
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===

Revision as of 03:43, 28 October 2020

GalU,glucose-1-phosphate uridylyltransferase

UTP-glucose-1-phosphate uridylyltransferase (GalU) carries out a key step in the generation of UDP-D-glucuronate.GalU catalyzes the addition of UTP to α-D-glucose 1-phosphate to yield UDP-D-glucose,which is the substrate for cellulose synthetase complex to produce cellulose.

Characterized by XJTU-China 2020

We cloned and confirmed the target fragment by PCR. The length of our fragment is 981bp. The following results showed the successful result.

Figure 1:PCR confirmation of the target fragment.
T--XJTU-China--Bgimprove02.jpeg
T--XJTU-China--Bgimprove01.jpeg

the mRNA level of B.s GalU is ~2.4 fold higher than that of E.coli, indicating the significant improvement of enzyme expression and activity. However, due to the lower expression of another key enzyme PGM for EPS production in BE strain, the final EPS yield is lower than that of EE strain but still comparable. Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 69
    Illegal AgeI site found at 387
    Illegal AgeI site found at 510
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 783