Difference between revisions of "Part:BBa K750115"

 
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<partinfo>BBa_K750115 short</partinfo>
 
<partinfo>BBa_K750115 short</partinfo>
  
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This part contains pBADcIT(BBa_K750104),PtetcIT(BBa_K750102),PcIGLT(BBa_K750103). Adding arabinose or atc can repress the expression of GFP from PcIGLT. In our original thoughts, we need plenty kind of logic gates to achieve number-switching of 0,1,2. And we chose this logic gate for our project.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 18:20, 25 September 2012

pBADcIT-PtetcIT-PcIGLT: arabinose or atc->cI protein->reduction of gfp(lva)

This part contains pBADcIT(BBa_K750104),PtetcIT(BBa_K750102),PcIGLT(BBa_K750103). Adding arabinose or atc can repress the expression of GFP from PcIGLT. In our original thoughts, we need plenty kind of logic gates to achieve number-switching of 0,1,2. And we chose this logic gate for our project.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
    Illegal NheI site found at 1083
    Illegal NheI site found at 1106
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 65
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3686