Difference between revisions of "Part:BBa K907002"

Revision as of 09:19, 22 September 2012

Binary signal generator, RBS(reverse) - attB - Promoter - attP - RBS

Dual phase protein generator, RBS(reverse) - attB - Promoter - attP - RBS

This part is composed of 3 elements.
1. RBSs: BBa_B0034, upsteam one is reversed
2. Promoter: BBa_J23119, Bacterial constitutive promoter
3. att sites: Recognition site for Mycobacteriophage Bxb1 integrase/excisionase We designed this part to generate two different proteins one at a time.

KAIST Dual Phase protein generator default.png

KAIST Dual Phase protein generator inverted.png

At the first state, this device promotes the transcription and translation of downstream gene because of its promoter orientation. When Mycobacteriophage Bxb1 integrase recognizes and inverts the sequence flanking with attB and attP sequence, promoter orientation reversed. Then this device promotes the transcription and translation of upstream gene(must be reversed at the construction step).
This device is tested by GFP and mRFP attached construct. See BBa_K0907004 and BBa_K0907005

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 75
Illegal NheI site found at 98
21
INCOMPATIBLE WITH RFC[21]
Illegal XhoI site found at 157
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 22
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 42
Illegal BsaI.rc site found at 125

@@ Line 15: / Line 15: @@
 <br>
 <br>
-[[Image: KAIST_Dual_Phase_protein_generator_default.png |center|900px]]
+[[Image: KAIST_Dual_Phase_protein_generator_default.png |center|4500px]][[Image: KAIST_Dual_Phase_protein_generator_inverted.png |center|450px]]
-At the first state, this device promotes the transcription and translation of downstream gene because of its promoter orientation.
+At the first state, this device promotes the transcription and translation of downstream gene because of its promoter orientation. When Mycobacteriophage Bxb1 integrase recognizes and inverts the sequence flanking with attB and attP sequence, promoter orientation reversed. Then this device promotes the transcription and translation of upstream gene(must be reversed at the construction step).
-<br>
-<br>
-[[Image: KAIST_Dual_Phase_protein_generator_inverted.png |center|900px]]
-When Mycobacteriophage Bxb1 integrase recognizes and inverts the sequence flanking with attB and attP sequence, promoter orientation reversed. Then this device promotes the transcription and translation of upstream gene(must be reversed at the construction step).
 <br>
 This device is tested by GFP and mRFP attached construct. See BBa_K0907004 and BBa_K0907005