Difference between revisions of "Part:BBa K907002"
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We designed this part to generate two different proteins one at a time. | We designed this part to generate two different proteins one at a time. | ||
+ | |||
+ | <br> | ||
+ | <br> | ||
+ | [[Image: KAIST_Dual_Phase_protein_generator_default.png |center|900px]] | ||
At the first state, this device promotes the transcription and translation of downstream gene because of its promoter orientation. | At the first state, this device promotes the transcription and translation of downstream gene because of its promoter orientation. | ||
− | |||
− | This device is tested by GFP and mRFP attached construct. See | + | |
+ | <br> | ||
+ | <br> | ||
+ | [[Image: KAIST_Dual_Phase_protein_generator_inverted.png |center|900px]] | ||
+ | |||
+ | When Mycobacteriophage Bxb1 integrase recognizes and inverts the sequence flanking with attB and attP sequence, promoter orientation reversed. Then this device promotes the transcription and translation of upstream gene(must be reversed at the construction step). | ||
+ | <br> | ||
+ | This device is tested by GFP and mRFP attached construct. See BBa_K0907004 and BBa_K0907005 | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here |
Revision as of 09:08, 22 September 2012
Binary signal generator, RBS(reverse) - attB - Promoter - attP - RBS
Dual phase protein generator, RBS(reverse) - attB - Promoter - attP - RBS
This part is composed of 3 elements.
1. RBS: BBa_B0034, upsteam one is reversed
2. Promoter: BBa_J23119, Bacterial constitutive promoter
3. att site: Recognition site for Mycobacteriophage Bxb1 integrase/excisionase
We designed this part to generate two different proteins one at a time.
At the first state, this device promotes the transcription and translation of downstream gene because of its promoter orientation.
When Mycobacteriophage Bxb1 integrase recognizes and inverts the sequence flanking with attB and attP sequence, promoter orientation reversed. Then this device promotes the transcription and translation of upstream gene(must be reversed at the construction step).
This device is tested by GFP and mRFP attached construct. See BBa_K0907004 and BBa_K0907005
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 75
Illegal NheI site found at 98 - 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 157
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 22
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 42
Illegal BsaI.rc site found at 125