Difference between revisions of "Part:BBa K785000:Design"
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[[Image:Lov_fmn_binding.PNG|frame|left|Lov domain, shows the binding with ligand FMN]] | [[Image:Lov_fmn_binding.PNG|frame|left|Lov domain, shows the binding with ligand FMN]] | ||
| + | |||
| + | [[Image:TetR.png|frame|left|tetR domain, shows the binding between protein and DNA]] | ||
===References=== | ===References=== | ||
Revision as of 06:02, 22 September 2012
Lov-tetR (light sensor with Ptet repression) light->PtetO repressed
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 106
Illegal PstI site found at 160
Illegal PstI site found at 289
Illegal PstI site found at 626
Illegal PstI site found at 940
Illegal PstI site found at 1033 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 106
Illegal PstI site found at 160
Illegal PstI site found at 289
Illegal PstI site found at 626
Illegal PstI site found at 940
Illegal PstI site found at 1033 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 106
Illegal PstI site found at 160
Illegal PstI site found at 289
Illegal PstI site found at 626
Illegal PstI site found at 940
Illegal PstI site found at 1033 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 106
Illegal PstI site found at 160
Illegal PstI site found at 289
Illegal PstI site found at 626
Illegal PstI site found at 940
Illegal PstI site found at 1033
Illegal AgeI site found at 478 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
This part is designed for our biowave project. See more about Biowave visit our wiki:[http://2012.igem.org/Team:Fudan_Lux Fudan_Lux 2012]
Source
This coding sequence is form the Avena sativa phototrophin1(AsLOV2) and tetracycline resistance. The whole gene is synthesized by Invitrogen company.
Lov domain, shows the binding with ligand FMN
tetR domain, shows the binding between protein and DNA