Difference between revisions of "Part:BBa K823040"
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This part reverses the positive Input of the pBad Promoter (only short form without upstream Repressor binding site) induced by Arabinose to a negative output of the lacZalpha Reporter, by the translational inhibitory action of the small RNA RyhB to uof translationally fused to lacZalpha. | This part reverses the positive Input of the pBad Promoter (only short form without upstream Repressor binding site) induced by Arabinose to a negative output of the lacZalpha Reporter, by the translational inhibitory action of the small RNA RyhB to uof translationally fused to lacZalpha. | ||
| − | [[Image:Inverter_graph.png|300px]] | + | [[Image:Inverter_graph.png|300px]][[Image:Inverter Construct.png|300px]] |
This Image depicts the ONPG assay done with this part. It converts the positive Input of the pBAD promoter induced by Arabinose into an negative output, therefore declining Miller Units with rising Arabinose concentration (as Repressor binding sites are missing, the output without Arabinose is not so high, as Promoter is leaky). | This Image depicts the ONPG assay done with this part. It converts the positive Input of the pBAD promoter induced by Arabinose into an negative output, therefore declining Miller Units with rising Arabinose concentration (as Repressor binding sites are missing, the output without Arabinose is not so high, as Promoter is leaky). | ||
Revision as of 13:38, 26 September 2012
Inverter: pBad-RyhB-pLac(R0011)-uof-lacZalpha
This part reverses the positive Input of the pBad Promoter (only short form without upstream Repressor binding site) induced by Arabinose to a negative output of the lacZalpha Reporter, by the translational inhibitory action of the small RNA RyhB to uof translationally fused to lacZalpha.
This Image depicts the ONPG assay done with this part. It converts the positive Input of the pBAD promoter induced by Arabinose into an negative output, therefore declining Miller Units with rising Arabinose concentration (as Repressor binding sites are missing, the output without Arabinose is not so high, as Promoter is leaky).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 65
Illegal BamHI site found at 495 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]