Difference between revisions of "Part:BBa K895000"
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<partinfo>BBa_K895000 short</partinfo> | <partinfo>BBa_K895000 short</partinfo> | ||
− | The constitutive promoter region (103 bp) from the Escherichia coli K-12 tatABCD (twin-arginine translocase) operon. This is a constitutive promoter that gives good levels of expression in E. coli, but is not inducible or repressible. The promoter region is cloned as an EcoRI / PstI fragment into pSB1C3 following Biobrick Standard [10]. This is a corrected part that replaces the <partinfo>BBa_K562000</partinfo> biobrick. | + | The constitutive promoter region (103 bp) from the <i>Escherichia coli</i> K-12 <i>tatABCD</i> (twin-arginine translocase) operon. This is a constitutive promoter that gives good levels of expression in E. coli, but is not inducible or repressible. The promoter region is cloned as an EcoRI / PstI fragment into pSB1C3 following Biobrick Standard [10]. This is a corrected part that replaces the <partinfo>BBa_K562000</partinfo> biobrick. |
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Revision as of 19:22, 21 September 2012
Eco_Ptat2
The constitutive promoter region (103 bp) from the Escherichia coli K-12 tatABCD (twin-arginine translocase) operon. This is a constitutive promoter that gives good levels of expression in E. coli, but is not inducible or repressible. The promoter region is cloned as an EcoRI / PstI fragment into pSB1C3 following Biobrick Standard [10]. This is a corrected part that replaces the BBa_K562000 biobrick.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 98
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]