Difference between revisions of "Part:BBa K861029"

Latest revision as of 07:38, 22 September 2012

PfadR regulated S-FadE

FadE gene in S. enterica and in E.coli have similar function. Yet it is shown that S-FadE has far better efficiency compared to FadE in E.coli. The original S-FadE gene contains a PstI restriction site. This part has already been point mutated (The G at 1983bp was mutated to A). We assembled S-FadE with PfadR (BBa_K861060) hoping it will only be overexpressed when fatty acids concentration is high.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 462
Illegal AgeI site found at 2652
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 619
Illegal SapI.rc site found at 907
Illegal SapI.rc site found at 1168

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 __NOTOC__
 <partinfo>BBa_K861029 short</partinfo>
-FadE gene in S. enterica and in E.coli have similar function. Yet it is shown that S-FadE have far better efficiency compared to FadE in E.coli. The original S-FadE gene contains PstI restriction site. This part has already been point mutated (The G at 1983bp was mutated to A). We assebled S-FadE with PfadR (BBa_K861060) hoping it will only be overexpressed when fatty acids concentration is high.
+FadE gene in S. enterica and in E.coli have similar function. Yet it is shown that S-FadE has far better efficiency compared to FadE in E.coli. The original S-FadE gene contains a PstI restriction site. This part has already been point mutated (The G at 1983bp was mutated to A). We assembled S-FadE with PfadR (BBa_K861060) hoping it will only be overexpressed when fatty acids concentration is high.
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