Difference between revisions of "Part:BBa K750109"

 
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<partinfo>BBa_K750109 short</partinfo>
 
<partinfo>BBa_K750109 short</partinfo>
  
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This part contains RBS of 1.0 strength, cI(lva) producer double terminator and PcIGLT-pBADGLT(BBa_K750109).The GFP from PcIGLT will be produced from the beginning. When cI protein is added to the parts, promoter PcI will be activated and repress expression of GFP. And when arabinose is added, this part will work agian because the promoter pBAD is activated. When the part is ligated with a promoter, the cI producer can work so that make the whole device work as we designed.
  
 
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Revision as of 17:19, 25 September 2012

cI->GFP(lva)<-arabinose

This part contains RBS of 1.0 strength, cI(lva) producer double terminator and PcIGLT-pBADGLT(BBa_K750109).The GFP from PcIGLT will be produced from the beginning. When cI protein is added to the parts, promoter PcI will be activated and repress expression of GFP. And when arabinose is added, this part will work agian because the promoter pBAD is activated. When the part is ligated with a promoter, the cI producer can work so that make the whole device work as we designed.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2042
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1982
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1657
    Illegal BsaI.rc site found at 2717