Difference between revisions of "Part:BBa K784022"

Latest revision as of 19:34, 26 September 2012

Phage Lambda fragment 8 - lytic proteins

This bio-brick is one of eight correlated bio-bricks – each one contains a one fragment of the phage lambda CI875s7. The phages fragments created in order to allow manipulation in the phage genome by manipulation smaller fragments, and re-factor the complete 48kb genome after the manipulation.
Each fragment was inserted into pSB1C3_MCS plasmid (BBa_K784023), which was designed mainly for this purpose. The MCS contain PacI and SpeI recognition sites, which does not appear at the phages genome.
More information is available [http://2012.igem.org/Team:Technion/Project/Phage here].
Fragment 8 contains the coding regions for the phages lytic causing proteins S and R.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 2633

@@ Line 4: / Line 4: @@
 This bio-brick is one of eight correlated bio-bricks – each one contains a one fragment of the phage lambda CI875s7. The phages fragments created in order to allow manipulation in the phage genome by manipulation smaller fragments, and re-factor the complete 48kb genome after the manipulation. <br>
 Each fragment was inserted into pSB1C3_MCS plasmid [[Part:BBa_K784023|(BBa_K784023)]], which was designed mainly for this purpose. The MCS contain PacI and SpeI recognition sites, which does not appear at the phages genome. <br>
+More information is available [http://2012.igem.org/Team:Technion/Project/Phage here]. <br>
 Fragment 8 contains the coding regions for the phages lytic causing proteins S and R. <br>