Difference between revisions of "Part:BBa K750108"

 
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<partinfo>BBa_K750108 short</partinfo>
 
<partinfo>BBa_K750108 short</partinfo>
  
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This part contains PcIGLT(BBa_K750103) and pBADGLT(BBa_K750000). The GFP from PcIGLT will be produced from the beginning. When cI protein is added to the parts, promoter PcI will be activated and repress expression of GFP. And when arabinose is added, this part will work agian because the promoter pBAD is activated. In our project, sometimes we need the cell can be switched in the order of bright-dark-bright to be used in display more numbers. So we designed this part to solve this problem.
  
 
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Revision as of 17:09, 25 September 2012

PcIGLT-pBADGLT:GFP(lva) expression system controlled by cI protein or arabinose

This part contains PcIGLT(BBa_K750103) and pBADGLT(BBa_K750000). The GFP from PcIGLT will be produced from the beginning. When cI protein is added to the parts, promoter PcI will be activated and repress expression of GFP. And when arabinose is added, this part will work agian because the promoter pBAD is activated. In our project, sometimes we need the cell can be switched in the order of bright-dark-bright to be used in display more numbers. So we designed this part to solve this problem.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1104
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1044
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 719
    Illegal BsaI.rc site found at 1779