Difference between revisions of "Part:BBa K801076"

Revision as of 22:53, 26 September 2012

Yeast expression cassette for CaXMT1 and CaMXMT1

Yeast Expression Cassette for the enzymes 1 and 2 of the caffeine biosynthesis pathway

Expression of CaXMT1 is controlled by the yeast TEF2 promoter and the yeast ADH1 terminator.

Expression of CaMXMT1 is controlled by the yeast TEF1 promoter and the yeast ADH1 terminator.

This BioBrick is a generator for the first two enzymes envolved in caffeine biosynthesis: methylxanthosine N-methyltransferase 1 and 7- methylxanthine N-methyltransferase 1. It is made up of the two single generators for CaXMT1 and CaMXMT1, which means that CaXMT1 is regulated by the Tef2 promoter and CaMXMT1 is regulated by the Tef1 promoter. In both cases, the Adh1 terminator was used.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 3613
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 138
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 2385

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 Expression of CaMXMT1 is controlled by the yeast TEF1 promoter and the yeast ADH1 terminator.
+This BioBrick is a generator for the first two enzymes envolved in caffeine biosynthesis: methylxanthosine N-methyltransferase 1 and 7- methylxanthine N-methyltransferase 1. It is made up of the two single generators for [https://parts.igem.org/Part:BBa_K801073 CaXMT1] and [https://parts.igem.org/Part:BBa_K801074 CaMXMT1], which means that CaXMT1 is regulated by the Tef2 promoter and CaMXMT1 is regulated by the Tef1 promoter. In both cases, the Adh1 terminator was used.
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