Difference between revisions of "Part:BBa K737067"

Revision as of 11:29, 26 September 2012

pLux OmpR hybrid promoter

In order to optimize the function of the lux promoter, We creat a hybrid promoter which combine plux with OmpR, and in this case, plux can be lead to expression by AHL and obtain by phosphorylation of OmpR protein. According to the research of it before, we camp up with a program . Keep the sequencing of Lux Box, -35box and -10box. Then put the sequencing of C3 point of OmpR promoter into -35box and -10box.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 __NOTOC__
 <partinfo>BBa_K737067 short</partinfo>
-Positively regulated, OmpR-controlled promoter ligated with B0034 can be used to controll Genetic circuit by light with  light-dependent control element.This promoter is taken from the upstream region of ompC. Phosphorylated OmpR binds to the three operator sites and activates transcription.
+In order to optimize the function of the lux promoter, We creat a hybrid promoter which combine plux with OmpR, and in this case, plux can be lead to expression by AHL and obtain by phosphorylation of OmpR protein.
+According to the research of it before, we camp up with a program . Keep the sequencing of Lux Box, -35box and -10box. Then put the sequencing of C3 point of OmpR promoter into -35box and -10box.
 <!-- Add more about the biology of this part here