Difference between revisions of "Part:BBa K801091:Design"

 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K801091 short</partinfo>
 
<partinfo>BBa_K801091 short</partinfo>
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<partinfo>BBa_K801091 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K801091 SequenceAndFeatures</partinfo>
  
 +
 +
 +
Plasmid (template for PCR) provided by John A. Morgan, School of Chemical Engineering, Purdue University, West Lafayette, Indiana, USA
 +
 +
http://www.ncbi.nlm.nih.gov/nuccore/AX366866
 +
 +
PMID: 15932991
 +
<br>'''Keywords:'''
 +
<!--These keywords are necessary to find your part using a fulltext sarch.-->
 +
<!--keyword_1, keyword_2, keyword_3, keyword_4, keyword_5-->
 +
 +
<br>'''Abbreviations:'''
 +
<!--*used_abbreviation_1 = full_name_of_used_abbreviations_1-->
 +
<!--*used_abbreviation_2 = full_name_of_used_abbreviations_2-->
  
 
===Design Notes===
 
===Design Notes===
−
-
 
  
 +
'''Related BioBrick:'''
 +
<!--*Other versions:[https://parts.igem.org/wiki/index.php?title=Part:BBa_?????? BBa_??????: Name_of_part] -->
 +
<!--*Related BioBricks:[https://parts.igem.org/wiki/index.php?title=Part:BBa_?????? BBa_??????: Name_of_part]
  
 +
'''Cloning details:'''<br>
 +
<!--*Designed in RFC10/RFC23/RFC25/RFC25_N-part-->
 +
<!--*Mutation C889G to delete XbaI restriction site-->
 +
<!--*Truncation upstream/downstream compared to template, ?explanation?-->
 +
 +
'''Quality control measures:'''<br>
 +
<!--*Test digestion using ?enzyme1? & ?enzyme2?/Not yet performed-->
 +
<!--*Sequencing using primer ?primer_name?/Not yet sequenced-->
 +
<!--*Part was partly sequenced/Part was totally sequenced-->
 +
 +
'''Backbone:'''<br>
 +
<!--*Backbone name: pSB1C3'/?backbone_name?-->
 +
<!--*Resistance: Amp/Cp/Kan/Tet-->
 +
<!--*Copynumber: low/medium/high-->
 +
 +
'''Protein coding:'''<br>
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<!--*Protein: ?Name_of_gene_product? [Nucleotide 1 to ???]-->
 +
<!--*The protein has the amino acid replacements ???99??? to ???99???.-->
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<!--*The protein encoded is posttranslationally modified by ???.-->
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<!--*Tag: n-terminally fused/c-terminally fused His5/His6/Strep/Flag/other-->
 +
 +
'''Enzymatic activity:'''
 +
<!--none/EC-number ?.?.?.?-->
 +
 +
'''Cytotoxicity:'''<br>
 +
<!--none/not known/cytotoxic for ''organism name''-->
 +
 +
'''Safety notes:'''<br>
 +
<!--Known and anticipated sefety issues: none/health_risk/environmental_risk/other_risk-->
 +
<!--Known and anticipated security issues: none/other.-->
 +
 +
'''Intellectual property:'''
 +
<!--Information on patent situation.-->
 +
<!--Intellectual property claims made by the authors.-->
 +
 +
'''Corresponding part author/authors:'''
 +
<!--https://igem.org/User_Information.cgi?user_id=????/email-->
  
 
===Source===
 
===Source===
  
−
Plasmid (template for PCR) provided by John A. Morgan, School of Chemical Engineering, Purdue University, West Lafayette, Indiana, USA
+
'''Source:'''<br>
 +
<!--*Commercial system: plasmid name, system name, company name-->
 +
<!--*Plasmid: p???, provided by ?name_of_person?, ?institute/university?, ?country?-->
 +
<!--*Preexisting BioBrick ?Bba_number?-->
 +
<!--*cDNA Clone: ?clone_name?, ?company_name?-->
 +
<!--*Synthesized by ?company_name?.-->
  
−
http://www.ncbi.nlm.nih.gov/nuccore/AX366866
+
<!--'''Forward Primer:'''<br><code>5'- ??? - 3'</code><br>-->
 +
<!--'''Reverse Primer:'''<br><code>5'- ??? - 3'</code><br>-->
 +
 
 +
'''Organism:'''<br>
 +
<!--*Genesequence derived from ''?organism_name?''-->
 +
<!--*Codonoptimized for ''?organism_name?''-->
 +
<!--*Designed for the following Chassis: ''?organism-name?''-->
 +
<!--*Statement about functionality in other chassis.-->
  
−
PMID: 15932991
 
  
 
===References===
 
===References===
 +
<!-- Here you find templates to insert references to literature and different databases-->
 +
 +
'''Literature references:'''<br>
 +
<!--*[http://www.ncbi.nlm.nih.gov/pubmed/?PMID? '''Pubmed:''' ?Author(s)?, ?year?: ?title?]-->
 +
 +
'''Database references:'''<br>
 +
<!--*[http://www.ncbi.nlm.nih.gov/nuccore/?accessNr? '''GenBank''': ?title?]-->
 +
<!--*[http://www.ebi.ac.uk/interpro/IEntry?ac=?accessNr? '''Interpro''': ?title?]-->
 +
<!--*[http://www.uniprot.org/uniprot/?accessNr? '''Uniprot''': ?title?]-->
 +
<!--*[http://pfam.sanger.ac.uk/family/?accessNr? '''Pfam:''' ?title?]-->
 +
<!--*[http://www.rcsb.org/pdb/explore/explore.do?structureId=?accessNR? '''PDB:''' ?tile?]-->
 +
<!--*[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=?accessNr? '''Branda:''' ?title?]-->

Revision as of 21:46, 25 September 2012

phenylalanine ammonia lyase (PAL) coding region


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2090
    Illegal BamHI site found at 352
    Illegal XhoI site found at 403
    Illegal XhoI site found at 466
    Illegal XhoI site found at 484
    Illegal XhoI site found at 562
    Illegal XhoI site found at 763
    Illegal XhoI site found at 1006
    Illegal XhoI site found at 1753
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 922
    Illegal NgoMIV site found at 1168
    Illegal NgoMIV site found at 1336
    Illegal NgoMIV site found at 1479
    Illegal NgoMIV site found at 1813
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 463
    Illegal BsaI site found at 793
    Illegal BsaI site found at 799
    Illegal BsaI.rc site found at 1924
    Illegal SapI site found at 753


Plasmid (template for PCR) provided by John A. Morgan, School of Chemical Engineering, Purdue University, West Lafayette, Indiana, USA

http://www.ncbi.nlm.nih.gov/nuccore/AX366866

PMID: 15932991
Keywords:


Abbreviations:

Design Notes

Related BioBrick:

Quality control measures:

Backbone:

Protein coding:

Enzymatic activity:

Cytotoxicity:

Safety notes:

Intellectual property:

Corresponding part author/authors:

Source

Source:


Organism:


References

Literature references:

Database references: