Difference between revisions of "Part:BBa K861140:Experience"

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<li>Antisense CCTGCAGTACTAGTATCATTGTTGAGCCAAAGCCTG
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<li>Antisense CCTGCAGTACTAGTATTACTTCTTAATGCCCATCTCTTCT
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<br/><li>Sense　　CGAATTCTTCTAGAGATGAGTATCCTGACCCGGTGG
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<br/><li>Sense　　 CGAATTCTTCTAGAGATGGCTGCCATTAATACGAAAG
 
<br/>After amplifying by PCR, the DNA fragmentation was examined by agarose gel electrophoresis, the lads show that the sizes of the gene were correct.
 
<br/>After amplifying by PCR, the DNA fragmentation was examined by agarose gel electrophoresis, the lads show that the sizes of the gene were correct.
 
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Latest revision as of 06:33, 21 September 2012

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K861140

As for the genes we clone, there is no difference between E. coli str. K12 MG1655 and more available DH5α. we purified and amplified these genes from genome of Escherichia coli str. DH5α using PCR. The primers contain the standard restriction enzyme cutting sites. The sequences of the primers used are as below.

  • Antisense CCTGCAGTACTAGTATTACTTCTTAATGCCCATCTCTTCT
  • Sense　　 CGAATTCTTCTAGAGATGGCTGCCATTAATACGAAAG
    After amplifying by PCR, the DNA fragmentation was examined by agarose gel electrophoresis, the lads show that the sizes of the gene were correct.

    User Reviews

    UNIQf9ebe6d720a3a177-partinfo-00000001-QINU UNIQf9ebe6d720a3a177-partinfo-00000002-QINU