Difference between revisions of "Part:BBa K797002:Experience"

Revision as of 20:39, 26 September 2012

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Please enter how you used this part and how it worked out.

Applications of BBa_K797002

We modified torA signal which submitted by Canbrige 2011(BBa_K233307). BBa_K233307 don't contain RBS so we assembled torA and RBS avoiding to raise a stop codon. Then we made parts such as LacP-torA-GFP-DT and check its expression by observation through a confocal microscope. These pictures shows the result.

The left picture shows E.coli having LacP-torA-GFP-DT and stained with Hoechst, and the rigft picture shows the same sample under UV. The left picture indicated that we observed E.coli and the left picture indicated that GFP expressed. According to this result, we concluded that RBS in our torA signal worked.

User Reviews

UNIQb1fc8bdad7059e42-partinfo-00000000-QINU UNIQb1fc8bdad7059e42-partinfo-00000001-QINU

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 This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
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 ===Applications of BBa_K797002===
+We modified torA signal which submitted by Canbrige 2011(BBa_K233307).
+BBa_K233307 don't contain RBS so we assembled torA and RBS avoiding to raise a stop codon.
+Then we made parts such as LacP-torA-GFP-DT and check its expression by observation through a confocal microscope.
+These pictures shows the result.<br>
+[[Image:300px-F17822ea-b51e-49f5-aa44-8dc8638a1ff6.png|300px]]
+[[Image:300PX-~2.JPG|300px]]<br>
+The left picture shows E.coli having LacP-torA-GFP-DT and stained with Hoechst, and the rigft picture shows the same sample under UV.
+The left picture indicated that we observed E.coli and the left picture indicated that GFP expressed.
+According to this result, we concluded that RBS in our torA signal worked.<br>
 ===User Reviews===