Difference between revisions of "Part:BBa K743006"
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This plasmid allows the integration of a Kanamycin resistance casette followed by a double terminator in to Synechocystis chromosome, this allows for selection in this cyanobacteria. It has a Chloramphenicol resistance casette for selection in E.coli | This plasmid allows the integration of a Kanamycin resistance casette followed by a double terminator in to Synechocystis chromosome, this allows for selection in this cyanobacteria. It has a Chloramphenicol resistance casette for selection in E.coli | ||
− | The use of BBa_K743000 and BBa_K743001 as recombination sites has no phenotypic effects on the cells. | + | The use of [https://parts.igem.org/Part:BBa_K743000 BBa_K743000] and [https://parts.igem.org/Part:BBa_K743001 BBa_K743001] as recombination sites has no deleterious phenotypic effects on the cells. |
The plasmid is intended to be used as a starting backbone for further adition of dna parts by Gibson assembly. | The plasmid is intended to be used as a starting backbone for further adition of dna parts by Gibson assembly. | ||
It was used by [http://2012.igem.org/Team:UC_Chile UC_Chile 2012 team] to succesfully transform Synechocystis | It was used by [http://2012.igem.org/Team:UC_Chile UC_Chile 2012 team] to succesfully transform Synechocystis |
Revision as of 23:16, 20 September 2012
psb1C3_IntK recombination plasmid for Synechocystis PCC6803
This plasmid allows the integration of a Kanamycin resistance casette followed by a double terminator in to Synechocystis chromosome, this allows for selection in this cyanobacteria. It has a Chloramphenicol resistance casette for selection in E.coli The use of BBa_K743000 and BBa_K743001 as recombination sites has no deleterious phenotypic effects on the cells. The plasmid is intended to be used as a starting backbone for further adition of dna parts by Gibson assembly. It was used by [http://2012.igem.org/Team:UC_Chile UC_Chile 2012 team] to succesfully transform Synechocystis
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1774
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1774
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1774
Illegal XhoI site found at 2271 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1774
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1774
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 511