Difference between revisions of "Part:BBa K743000:Design"

Revision as of 14:53, 20 September 2012

Synechocystis PCC6803 neutral recombination site.

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI site found at 511

Design Notes

According to literature, in Synechocystis the recombination sites must be at least 450bp long to allow a rasonably high transformation efficiency, being 700bp the optimum size.

Source

PCR amplified from synechocystis PCC6803 chromosome, orf slr0370.

References

http://genome.kazusa.or.jp/cyanobase/Synechocystis

Revision as of 14:50, 20 September 2012 (view source) Juan Alamos (Talk \| contribs) (→‎Source) ← Older edit		Revision as of 14:53, 20 September 2012 (view source) Juan Alamos (Talk \| contribs) (→‎References) Newer edit →
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	===References===		===References===
		+	http://genome.kazusa.or.jp/cyanobase/Synechocystis