Difference between revisions of "Part:BBa K812300"

 
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<partinfo>BBa_K812300 short</partinfo>
 
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To use this plasmid, you simply have to insert your protein between the EcoRI and PstI site. It is better to add a kozac sequence in front of your gene to enhance its expression.
 
To use this plasmid, you simply have to insert your protein between the EcoRI and PstI site. It is better to add a kozac sequence in front of your gene to enhance its expression.
 
This plasmid contains the standard registry insert J04450 as a negative cloning control.
 
This plasmid contains the standard registry insert J04450 as a negative cloning control.
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The Heat Shock Promoter 70 induce the expression of the protein which is after HSP70. The induction can occur heating tadpole from 21°C to 34°C.
  
 
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Revision as of 15:19, 20 September 2012

Biobricked pCS2+ plasmid with HSP70 promoter for frogs and chicken

This plasmid is a biobricked version of the plasmid pSC2+(K812000)with HSP70 promoter. This plasmid is made of a bacterial ampicilin resistant backbone. The Biobrick site is surrounded by a 5'UTR expression enhancer, and a poly-A tail with a SV40 3' UTR that enhance the stabilisation of the mRNA and the expression of the protein.

To use this plasmid, you simply have to insert your protein between the EcoRI and PstI site. It is better to add a kozac sequence in front of your gene to enhance its expression. This plasmid contains the standard registry insert J04450 as a negative cloning control.

The Heat Shock Promoter 70 induce the expression of the protein which is after HSP70. The induction can occur heating tadpole from 21°C to 34°C.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3570
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 248
    Illegal NotI site found at 3576
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3570
    Illegal BglII site found at 3407
    Illegal BamHI site found at 3555
    Illegal XhoI site found at 3126
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 3570
    Illegal suffix found at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 3570
    Plasmid lacks a suffix.
    Illegal XbaI site found at 3585
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NgoMIV site found at 2611
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI.rc site found at 1634
    Illegal SapI site found at 551
    Illegal SapI.rc site found at 3419