Difference between revisions of "Part:BBa K863121:Design"

(→‎Design Notes)
(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
 
Added Freiburg-restriction-sites for in frame assembly and a His-tag.
 
Added Freiburg-restriction-sites for in frame assembly and a His-tag.
 +
 +
Primers that were used to make this BioBrick:
 +
{|
 +
|GFP_Frei||TACGGAATTCGCGGCCGCTTCTAGATGGCCGGCATGCGTAAAGGAGAAGAACTT
 +
|-
 +
|GFP_His6_compl||CTGCAGCGGCCGCTACTAGTATTAACCGGTGTGATGGTGATGGTGATGTTTGTATAGTTCATCCATGCCATGTG
 +
|}
  
 
===Source===
 
===Source===

Revision as of 19:42, 26 September 2012

green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (His-tag)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 4
    Illegal AgeI site found at 742
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 653


Design Notes

Added Freiburg-restriction-sites for in frame assembly and a His-tag.

Primers that were used to make this BioBrick:

GFP_Frei TACGGAATTCGCGGCCGCTTCTAGATGGCCGGCATGCGTAAAGGAGAAGAACTT
GFP_His6_compl CTGCAGCGGCCGCTACTAGTATTAACCGGTGTGATGGTGATGGTGATGTTTGTATAGTTCATCCATGCCATGTG

Source

BBa_I13522

References