Difference between revisions of "Part:BBa K863102:Design"

 
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===Design Notes===
 
===Design Notes===
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You can find additional info to the Cellulose binding domain here: [https://parts.igem.org/Part:BBa_K863101:Design CBDcex_Freiburg]
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The Biobrick was designed to express the cellulose binding domain of ''Cellulomonas fimi'' exoglucanase (CBDcex) under the control of a T7-promoter. 12 additional conserved bases (4 AS) were isolated upstream of the cellulose binding domain and 9 bases downstream of the exoglucanase gene and also a short C-terminal (Glycine, Serine) linker was added. The sequence ends with the ''Age''I-restriction-site of the Freiburg-Suffix, to make in frame assembly possible.
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===Source===
 
===Source===

Revision as of 23:19, 25 September 2012

Cellulose binding Domain of C. Fimi Exoglucanase with T7, RBS, GS-Linker (Freiburg-Standard)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 365
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The Biobrick was designed to express the cellulose binding domain of Cellulomonas fimi exoglucanase (CBDcex) under the control of a T7-promoter. 12 additional conserved bases (4 AS) were isolated upstream of the cellulose binding domain and 9 bases downstream of the exoglucanase gene and also a short C-terminal (Glycine, Serine) linker was added. The sequence ends with the AgeI-restriction-site of the Freiburg-Suffix, to make in frame assembly possible.

Source

Cloning-vector

References