Difference between revisions of "Part:BBa K769201:Experience"

 
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===Applications of BBa_K769201===
 
===Applications of BBa_K769201===
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===2015 LZU-China===
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In iGEM 2015, LZU-China used the part as a new reporting gene to monitor the content of pollution. We submitted a twins part [http：//parts.igem.org/Part:BBa_K1755001 BBa_K1755001].
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It can increase the electric output by synthesis of riboflavin.
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Besides, riboflavin itself is yellow.
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After the addition of ribB, the bacteria will be yellow.
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== Usage in MFC ==
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[[File:IGEM_2015_MFC.JPG|300px]]
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Microbial Fuel Cells (MFCs) which can convert contaminants in wastewaters into energy is an ideal approach to solve both pollution problem and energy crisis. However, MFCs is hard to determine the contaminants concentrations which is a major drawback for MFCs applications, because of the electric output is too low.
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Riboflavin is an excellent electron transport complex. However, the whole sequence of riboflavin synthase is too huge(more than 3000bp) to link to the plasmid pSB1C3. Evidence shows that, part of the riboflavin synthase DNA sequence (ribB) can also improve the secretion of riboflavin.
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[[File:LZU_iGEM_2015_ribB_picture.JPG|400px]]
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Figure 1:Normal E.coli(left one)and E.coli with ribB(right one)
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K1755001 SequenceAndFeatures</partinfo>
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<!-- Uncomment this to enable Functional Parameter display
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===Functional Parameters===
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<partinfo>BBa_K1755001 parameters</partinfo>
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<!-- -->
  
 
===User Reviews===
 
===User Reviews===

Revision as of 01:25, 13 September 2015


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K769201

2015 LZU-China

In iGEM 2015, LZU-China used the part as a new reporting gene to monitor the content of pollution. We submitted a twins part [http：//parts.igem.org/Part:BBa_K1755001 BBa_K1755001].

It can increase the electric output by synthesis of riboflavin.

Besides, riboflavin itself is yellow. After the addition of ribB, the bacteria will be yellow.

Usage in MFC

IGEM 2015 MFC.JPG

Microbial Fuel Cells (MFCs) which can convert contaminants in wastewaters into energy is an ideal approach to solve both pollution problem and energy crisis. However, MFCs is hard to determine the contaminants concentrations which is a major drawback for MFCs applications, because of the electric output is too low.

Riboflavin is an excellent electron transport complex. However, the whole sequence of riboflavin synthase is too huge(more than 3000bp) to link to the plasmid pSB1C3. Evidence shows that, part of the riboflavin synthase DNA sequence (ribB) can also improve the secretion of riboflavin.

LZU iGEM 2015 ribB picture.JPG Figure 1:Normal E.coli(left one)and E.coli with ribB(right one)

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 387
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 277
    Illegal AgeI site found at 319
  • 1000
    COMPATIBLE WITH RFC[1000]


User Reviews

UNIQf4db0b303636f336-partinfo-00000001-QINU UNIQf4db0b303636f336-partinfo-00000002-QINU