Difference between revisions of "Part:BBa K777121:Design"
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===References=== | ===References=== | ||
* Paul K., Nieto V., Carlquist W.C., Blair D.F., Harshey R.M. The c-di-GMP Binding Protein YcgR Controls Flagellar Motor Direction and Speed to Affect Chemotaxis by a “Backstop Brake” Mechanism. Mol Cell. 2010. 38: 128–139. | * Paul K., Nieto V., Carlquist W.C., Blair D.F., Harshey R.M. The c-di-GMP Binding Protein YcgR Controls Flagellar Motor Direction and Speed to Affect Chemotaxis by a “Backstop Brake” Mechanism. Mol Cell. 2010. 38: 128–139. | ||
| + | * Ko M., Park C. 2000. Two novel flagellar components and H-NS are involved in the motor function of Escherichia coli. J. Mol. Biol. 303: 371-382. | ||
Latest revision as of 14:23, 25 September 2012
yhjH
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
- yhjH was amplified from genomic DNA of E. coli DH10B via PCR using the following primers:
- Fwd: 5'-gtttcttcgaattcgcggccgcttctagatgataaggcaggttatccagc-3'
- Rev: 5'-gtttcttcctgcagcggccgctactagtattatagcgccagaaccgccgtattcagc-3'
Source
- The part was amplified from genomic DNA of E. coli str. K-12 substr. DH10B, complete genome (CP000948.1).
References
- Paul K., Nieto V., Carlquist W.C., Blair D.F., Harshey R.M. The c-di-GMP Binding Protein YcgR Controls Flagellar Motor Direction and Speed to Affect Chemotaxis by a “Backstop Brake” Mechanism. Mol Cell. 2010. 38: 128–139.
- Ko M., Park C. 2000. Two novel flagellar components and H-NS are involved in the motor function of Escherichia coli. J. Mol. Biol. 303: 371-382.