Difference between revisions of "Part:BBa K777117:Design"
(→‎Design Notes)
 
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===Design Notes===
 
===Design Notes===
 
* ''motB'' was amplified from genomic DNA of ''E. coli'' DH10B via PCR using the following primers:  
 
* ''motB'' was amplified from genomic DNA of ''E. coli'' DH10B via PCR using the following primers:  
−
** Fwd: gtttcttcgaattcgcggccgcttctagatgaagaatcaagcgcatcc
+
** Fwd: 5'-gtttcttcgaattcgcggccgcttctagatgaagaatcaagcgcatcc-3'
−
** Rev: gtttcttcctgcagcggccgctactagtatcacctcggttcggctgatgg
+
** Rev: 5'-gtttcttcctgcagcggccgctactagtatcacctcggttcggctgatgg-3'
  
 
===Source===
 
===Source===

Latest revision as of 08:43, 19 September 2012

motB


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 310


Design Notes

  • motB was amplified from genomic DNA of E. coli DH10B via PCR using the following primers:
    • Fwd: 5'-gtttcttcgaattcgcggccgcttctagatgaagaatcaagcgcatcc-3'
    • Rev: 5'-gtttcttcctgcagcggccgctactagtatcacctcggttcggctgatgg-3'

Source

  • The part was amplified from genomic DNA of E. coli str. K-12 substr. DH10B, complete genome (CP000948.1).

References