Difference between revisions of "Part:BBa K914000"

 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K914000 short</partinfo>
 
<partinfo>BBa_K914000 short</partinfo>
  
 
This part is composed of a tRNA amber suppressor, supD (K228001), which is under the control of pLac (R0011). A terminator is added (B1006, but the sequence is not the exact one, it's not a problem here).
 
This part is composed of a tRNA amber suppressor, supD (K228001), which is under the control of pLac (R0011). A terminator is added (B1006, but the sequence is not the exact one, it's not a problem here).
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This part is used to allow the expression of gene that have one or many amber codon instead of a Serine. Adding IPTG could be usefull when there are to many amber codon in the cell.
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This part is used to allow the expression of gene that have one or many amber codon instead of a Serine.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 00:13, 21 September 2012

pLac-supD-T

This part is composed of a tRNA amber suppressor, supD (K228001), which is under the control of pLac (R0011). A terminator is added (B1006, but the sequence is not the exact one, it's not a problem here). This part is used to allow the expression of gene that have one or many amber codon instead of a Serine.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 96
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 99