Difference between revisions of "Part:BBa K861024"

 
Line 1: Line 1:
−
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K861024 short</partinfo>
 
<partinfo>BBa_K861024 short</partinfo>
  
−
IPTG induced FadE protein. FadE is originated from e.coli DH5&#945;, catalyzing the conversion of acyl-CoA to enoyl-CoA.
+
IPTG induced FadE protein. To find the optimal combination of those fatty acid degradation enzymes, we used IPTG induced promoter BBa_R0011 to express and extract those proteins, put them in different combination with substrates needed for fatty acid oxidation in a test tube to look for the combination that have best degradation capability. FadE is originated from e.coli DH5&#945;, catalyzing the conversion of acyl-CoA to enoyl-CoA.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 17:21, 18 September 2012

IPTG induced FadE

IPTG induced FadE protein. To find the optimal combination of those fatty acid degradation enzymes, we used IPTG induced promoter BBa_R0011 to express and extract those proteins, put them in different combination with substrates needed for fatty acid oxidation in a test tube to look for the combination that have best degradation capability. FadE is originated from e.coli DH5α, catalyzing the conversion of acyl-CoA to enoyl-CoA.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 668
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 451
    Illegal SapI.rc site found at 1324
    Illegal SapI.rc site found at 2467