Difference between revisions of "Part:BBa K777109:Design"

 
Line 1: Line 1:
−
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K777109 short</partinfo>
 
<partinfo>BBa_K777109 short</partinfo>
Line 8: Line 7:
 
===Design Notes===
 
===Design Notes===
 
* The ''fliC'' gene contained three PstI sites and one SpeI site that had to be removed. These mutations were induced with different primers via overlap-PCR.
 
* The ''fliC'' gene contained three PstI sites and one SpeI site that had to be removed. These mutations were induced with different primers via overlap-PCR.
−
* ''flhDC'' was amplified from genomic DNA of E. coli DH10B via PCR using the following primers:  
+
* ''fliC'' was amplified from genomic DNA of ''E. coli'' DH10B via PCR using the following primers:  
−
** Fwd: ACTGAATTCGCGGCCGCTTCTAGATGCATACCTCCGAGTTGCTG
+
** Fwd: GTTTCTTCGAATTCGCGGCCGCTTCTAGATGGCACAAGTCATTAATACC
−
** Rev: TCCTGCAGCGGCCGCTACTAGTTACTGCCCGGGATGGCGGTTGACATAAGCcGCAGGCAAAGCTGCCAACAG
+
** Rev: GTTTCTTCGAATTCGCGGCCGCTTCTAGTTAACCCTGgAGCAGAGaCAGAACC <br> (the uncapitalized "g" induces the mutation for removal of the third PstI site)
  
  
Line 16: Line 15:
 
===Source===
 
===Source===
  
−
* The part was amplified from genomic DNA of E. coli str. K-12 substr. DH10B, complete genome (CP000948.1).
+
* The part was amplified from genomic DNA of ''E. coli'' str. K-12 substr. DH10B, complete genome (CP000948.1).
  
 
===References===
 
===References===

Revision as of 15:22, 18 September 2012

fliC


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1224
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 301
    Illegal AgeI site found at 709
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

  • The fliC gene contained three PstI sites and one SpeI site that had to be removed. These mutations were induced with different primers via overlap-PCR.
  • fliC was amplified from genomic DNA of E. coli DH10B via PCR using the following primers:
    • Fwd: GTTTCTTCGAATTCGCGGCCGCTTCTAGATGGCACAAGTCATTAATACC
    • Rev: GTTTCTTCGAATTCGCGGCCGCTTCTAGTTAACCCTGgAGCAGAGaCAGAACC
      (the uncapitalized "g" induces the mutation for removal of the third PstI site)


Source

  • The part was amplified from genomic DNA of E. coli str. K-12 substr. DH10B, complete genome (CP000948.1).

References