Difference between revisions of "Part:BBa K817018"
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These cassette encodes an autoregulated resolvase(serine type recombinase)from E.coli F plasmid transposon tn1000(tn3 family). Its promoter region consists of 3 sub-sites(res site) and can process recombination. This cassette can resolve multimer that formed during replicative transposition, so it can also resolve plasmid multimer providing plasmid stability by avoiding dimer catastrophe. For this reason, it is multimer resolution system(MRS) that provide the same function as E.coli chromosome XerCD/dif but acts independent of cell cycle and may have higher efficiency on plasmids compared with slow XerCD system. | These cassette encodes an autoregulated resolvase(serine type recombinase)from E.coli F plasmid transposon tn1000(tn3 family). Its promoter region consists of 3 sub-sites(res site) and can process recombination. This cassette can resolve multimer that formed during replicative transposition, so it can also resolve plasmid multimer providing plasmid stability by avoiding dimer catastrophe. For this reason, it is multimer resolution system(MRS) that provide the same function as E.coli chromosome XerCD/dif but acts independent of cell cycle and may have higher efficiency on plasmids compared with slow XerCD system. | ||
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| − | [[Image:NTU-Taida-Mrs-3.png]] | + | [[Image:NTU-Taida-Mrs-3.png|600px|center]] |
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Revision as of 11:20, 18 September 2012
Phs-GFP
These cassette encodes an autoregulated resolvase(serine type recombinase)from E.coli F plasmid transposon tn1000(tn3 family). Its promoter region consists of 3 sub-sites(res site) and can process recombination. This cassette can resolve multimer that formed during replicative transposition, so it can also resolve plasmid multimer providing plasmid stability by avoiding dimer catastrophe. For this reason, it is multimer resolution system(MRS) that provide the same function as E.coli chromosome XerCD/dif but acts independent of cell cycle and may have higher efficiency on plasmids compared with slow XerCD system.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]