Difference between revisions of "Part:BBa K812012"

 
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<partinfo>BBa_K812012 short</partinfo>
 
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This part contains 3 myc domain for protein staining using antiMyc antibodies
 
This part contains 3 myc domain for protein staining using antiMyc antibodies
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This part can be micro-injected using pSC2+ ( <html><a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K812000">BBa_K812000</a></html>) or related plasmid as a backbone.
  
 
<!-- Add more about the biology of this part here
 
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Revision as of 23:39, 26 September 2012

OsTirI Ubiquitinase E3 for AID tagged protein degradation in the presence of auxin

OsTiRI is an ubuquitinase E3 that recognise and trigger the ubiquitination of every AID tagged protein. This part works with K812011 which is a AID tagged GFP. OsTirI is a protein comming from rice that have been identified as working in mammalian cells and yeast.

This part is a part of a device pattented by Kanemaki Masato, Kakimoto Tatsuo, Nishimura Kohei, Takisawa Haruhiko and Fukagawa Tatsuo for yeast and mammalian cells use (http://www.freepatentsonline.com/y2012/0115232.html). However the pattent does not cover the use for oviparian such as frogs and chicken.

This part contains 3 myc domain for protein staining using antiMyc antibodies

This part can be micro-injected using pSC2+ ( BBa_K812000) or related plasmid as a backbone.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 433
    Illegal NotI site found at 287
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1648
    Illegal XhoI site found at 356
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 55
    Illegal NgoMIV site found at 164
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 79