Difference between revisions of "Part:BBa K777113:Design"
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===Design Notes=== | ===Design Notes=== | ||
*''motA'' was amplified from genomic DNA of ''E. coli'' DH10B via PCR using the following primers: | *''motA'' was amplified from genomic DNA of ''E. coli'' DH10B via PCR using the following primers: | ||
| − | **Fwd: | + | **Fwd: gtttcttcgaattcgcggccgcttctagatgaccggtggaagccttgg |
| − | **Rev: | + | **Rev: gtttcttcctgcagcggccgctactagtatcatgcttcctcggttgtcg |
| − | + | ||
| − | + | ||
===Source=== | ===Source=== | ||
Revision as of 08:33, 19 September 2012
motA
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 266
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 4
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 772
Design Notes
- motA was amplified from genomic DNA of E. coli DH10B via PCR using the following primers:
- Fwd: gtttcttcgaattcgcggccgcttctagatgaccggtggaagccttgg
- Rev: gtttcttcctgcagcggccgctactagtatcatgcttcctcggttgtcg
Source
- The part was amplified from genomic DNA of E. coli str. K-12 substr. DH10B, complete genome (CP000948.1).