Difference between revisions of "Part:BBa K819017:Design"

 
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<partinfo>BBa_K819017 short</partinfo>
 
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===Design Notes===
 
===Design Notes===
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SulA promoter in 408 form.
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The original SulA with the binding site of CTGT(N)<sub>8</sub>ACAG is mutated to CCGT(N)<sub>8</sub>ACGG, which is the 408 form.<br />The LexA 408 protein has corresponding mutations of PA40, NS41 and AS42.
  
  

Revision as of 16:38, 25 September 2012

Luminesensor repressible SulA408 promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The original SulA with the binding site of CTGT(N)8ACAG is mutated to CCGT(N)8ACGG, which is the 408 form.
The LexA 408 protein has corresponding mutations of PA40, NS41 and AS42.


Source

SulA promoter sequence is got from regulon.org.

References